p smad2 3 rabbit anti human polyclonal antibody  (Cell Signaling Technology Inc)

Journal: Immunology

Article Title: High Smad7 sustains inflammatory cytokine response in refractory coeliac disease

doi: 10.1111/imm.12690

Figure Lengend Snippet: Transforming growth factor‐β 1 (TGF‐β 1)/Smad signalling is altered in refractory coeliac disease (RCD). Representative photomicrographs (× 100 original magnification) of p‐Smad2/3‐stained paraffin‐embedded sections of biopsy samples taken from one control (CTR), one patient with active coeliac disease (ACD), one patient with inactive coeliac disease (ICD) and one patient with RCD. In RCD, p‐Smad2/3 staining is reduced in both the epithelial and lamina propria compartments. The right panel shows the number of pSmad2/3‐positive cells per high‐power field (hpf) in duodenal sections taken from five CTR, three patients with ACD, three patients with ICD and six patients with RCD (three RCDI and three RCDII). Data indicate mean ± SE. [Colour figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Immunohistochemistry Paraffin‐embedded duodenal sections of four patients with ACD, four patients with ICD, seven patients with RCD (four RCDI, three RCDII) and four healthy controls were analysed for Smad7 and p‐Smad2/3 using anti‐human Smad7 mouse monoclonal antibody (R&D Systems, Minneapolis, MN: 1 : 50 final dilution) or anti‐human p‐Smad2/3 rabbit polyclonal antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA; 1 : 1000 final dilution).

Techniques: Staining